Transcriptome of sorted ILC2s in mice fed high-fiber and control diet. Transcriptome of sorted ILC2s in mice fed high-fiber and control diet

To determine the activation patterns of ILC2s and associated ILC2-intrinsic functional molecules triggered by the high fiber diet, we administered either a control or high fiber diet in WT mice and performed RNA sequencing of FACS-sorted ILC2s from mouse colons. RNAseq libraries were prepared from 1,000 sorted colonic lamina propria ILC2s (CD45+Lin-CD90.2+CD127+KLRG1+) by the Epigenomics Core at WCM using the Clontech SMARTer® Ultra® Low Input RNA Kit V4 (Clontech Laboratories). Libraries were sequenced on an Illumina HiSeq 2500, generating 50 bp single-end reads. Two samples from two control diet-fed WT SPF mice and two samples from two high fiber diet-fed WT SPF mice were used. Overall design: 2 samples were used for each diet group, each sample representing one mouse.

为探究高纤维饮食诱导的2型固有淋巴细胞（ILC2）及其固有功能性相关分子的激活模式，我们对野生型（Wild Type, WT）小鼠分别饲喂对照饮食与高纤维饮食，并对从小鼠结肠中经荧光激活细胞分选（Fluorescence Activated Cell Sorting, FACS）得到的ILC2进行RNA测序。本研究的RNA测序文库由威尔康奈尔医学院（Weill Cornell Medicine, WCM）表观基因组学核心实验室使用Clontech SMARTer® Ultra® 低起始量RNA试剂盒V4（Clontech Laboratories），从1000个分选得到的结肠固有层ILC2（CD45+Lin-CD90.2+CD127+KLRG1+）制备而成。文库在Illumina HiSeq 2500测序平台上进行测序，生成50 bp单端读段。本实验共使用4个样本：2个样本来自2只饲喂对照饮食的野生型无特定病原体（Specific Pathogen Free, SPF）小鼠，另外2个样本来自2只饲喂高纤维饮食的野生型无特定病原体（Specific Pathogen Free, SPF）小鼠。实验总体设计：每个饮食组设置2个生物学重复样本，每个样本对应1只小鼠。