Comparison of gene expression signature between nascent Lhx6-GFP+ cells from mouse embryonic brain and differentiated Lhx6-GFP+ cells from ES cells (as well as comparing those of Lhx6-GFP+ and Lhx6-GFP- cells from ES cells). Mus musculus

There was a remarkable similarity in the molecular properties of the MGE-GFP+ and ES-GFP+ cells. In particular, genes that are important for medial ganglionic eminence (MGE) and cortical interneurons development are both high in expression in both MGE-Lhx6-GFP+ and ES-Lhx6-GFP+ cells (compared to ES-Lhx6-GFP- cells). Overall design: To investigate how closely ES cells-derived Lhx6-GFP+ cells resembled authentic Lhx6+ MGE cells, and to define the molecular properties of the Lhx6-GFP+ and Lhx6-GFP- cells from differentiated ES cells, we compared their gene expression profiles. We used FACS to purify GFP+ cells from the E12.5 MGE of Lhx6-GFP transgenic mice. ES-Lhx6-GFP+ cells and ES-Lhx6-GFP- cells (both from D12 EB aggregates) were also isolated by fluorescent activated cell sorting (FACS) and all of the RNA samples were subjected to RNA expression microarray analyses.

MGE-GFP+细胞与ES-GFP+细胞的分子特性具有显著相似性。特别值得注意的是，对内侧神经节隆起（medial ganglionic eminence, MGE）及皮层中间神经元发育至关重要的基因，在MGE-Lhx6-GFP+与ES-Lhx6-GFP+细胞中均呈高表达（相较于ES-Lhx6-GFP-细胞）。实验总体设计：为探究胚胎干细胞（embryonic stem, ES）来源的Lhx6-GFP+细胞与天然Lhx6+ MGE细胞的相似程度，并明确分化所得ES细胞中Lhx6-GFP+与Lhx6-GFP-细胞的分子特性，我们对二者的基因表达谱进行了比较分析。我们采用荧光激活细胞分选（fluorescence activated cell sorting, FACS）技术，从Lhx6-GFP转基因小鼠的E12.5龄内侧神经节隆起组织中纯化得到GFP+细胞。同时，我们亦通过FACS分离得到源自D12胚体（embryoid body, EB）聚集物的ES-Lhx6-GFP+细胞与ES-Lhx6-GFP-细胞；所有RNA样本均进行了RNA表达微阵列分析。