Table_1_Mesenchymal stem cells therapy improves ovarian function in premature ovarian failure: a systematic review and meta-analysis based on preclinical studies.doc

BackgroundStudies have revealed that the transplantation of mesenchymal stem cells (MSCs) might be a potential star candidate for premature ovarian failure (POF) in animal experiments. However, individual studies with a small sample size cannot be used to draw a clear conclusion. Therefore, we conducted a systematic review and meta-analysis to explore the potential of using MSCs in the treatment of POF in animals.
MethodsSeven databases were searched for studies exploring the effect of the transplantation of MSCs on POF in animal models. The PRISMA guideline was followed, and the methodological quality was ensured using SYRCLE’s risk of bias tool. RevMan 5.4 and STATA 12.0 software was performed to meta-analysis.
ResultsIn total, 37 studies involving 1,079 animals were included. Significant associations were found for MSCs with the levels of E2 (SMD 2.69 [95% CI 1.97, 3.41]), FSH (-2.02, [-2.74, -1.30]), primary follicles (2.04, [1.17, 2.92]), secondary follicles (1.93, [1.05, 2.81]), and primordial follicles (2.38, [1.19, 3.57]. Other outcomes, such as AMH, LH, INHB, antral follicles, growing follicles, mature follicles, and early antral were also found to be significant. There was no difference in FSH/LH, corpus leteum, follicles, and estruc cycle.
ConclusionsOur meta-analysis result indicated that the transplantation of MSCs might exert therapeutic effects on animal models of POF, and these effects might be associated with improving the disorder of the sexual cycle, modulating serum hormone expressions to a better state, and restoring ovarian function.

背景 既往研究显示，在动物实验中，间充质干细胞（mesenchymal stem cells, MSCs）移植有望成为早发性卵巢功能不全（premature ovarian failure, POF）的潜在治疗候选方案。然而，单一样本量较小的单项研究无法得出明确结论。因此，本研究开展系统评价与荟萃分析，以探讨MSCs在动物POF模型治疗中的应用潜力。

方法 我们检索了7个数据库，纳入探讨MSCs移植对动物POF模型影响的相关研究。本研究遵循PRISMA报告规范，并采用SYRCLE偏倚风险工具评估方法学质量。使用RevMan 5.4与STATA 12.0软件进行荟萃分析。

结果 最终纳入37项研究，共涉及1079只实验动物。分析显示，MSCs移植与雌二醇（E2）水平（标准化均数差SMD=2.69，95%置信区间CI：1.97~3.41）、卵泡刺激素（FSH）水平（SMD=-2.02，95%CI：-2.74~-1.30）、初级卵泡（primary follicles）数量（SMD=2.04，95%CI：1.17~2.92）、次级卵泡（secondary follicles）数量（SMD=1.93，95%CI：1.05~2.81）以及原始卵泡（primordial follicles）数量（SMD=2.38，95%CI：1.19~3.57）均存在显著关联。其余结局指标，如抗缪勒管激素（AMH）、黄体生成素（LH）、抑制素B（INHB）、窦状卵泡、生长卵泡、成熟卵泡及早期窦状卵泡等，同样具有统计学显著性。而FSH/LH比值、黄体、卵泡总数及发情周期未观察到显著差异。

结论 本荟萃分析结果表明，MSCs移植可对POF动物模型发挥治疗作用，其机制可能与改善生殖周期紊乱、调节血清激素水平至正常状态以及恢复卵巢功能有关。