eRNAs and super-enhancer lncRNAs are functional in human prostate cancer. eRNAs and super-enhancer lncRNAs are functional in human prostate cancer

Prostate cancer (PCa) is one of the most commonly diagnosed cancers in males worldwide. lncRNAs (long non-coding RNAs) play a significant role in the occurrence and development of PCa. eRNAs (enhancer RNA) and SE-lncRNAs (super-enhancer lncRNA) are important elements of lncRNAs, but the role of eRNAs and SE-lncRNAs in PCa remains largely unclear. In this work, we identified 681 eRNAs and 292 SE-lncRNAs that were expressed differentially in PCa using a microarray. We constructed a transcriptional regulation network that eRNA related enhancer and the target genes shared the same TF binding motifs. Further, we investigated whether CTCF played a role in mediating the transcriptional regulation network. eRNAs, especially those that regulate androgen response genes, may be candidates for prognostic biomarkers and therapy targets. Our work provide a new perspective for developing medical treatments and therapies for prostate cancer. Overall design: In the study presented here, three prostate tumors and corresponding adjacent normal prostate tissues was used to acquire expression profiles of a total of 18793 coding genes and 72002 lncRNAs, leading to the successful construction of supervised

前列腺癌（Prostate cancer, PCa）是全球范围内男性最常见的确诊癌症之一。长链非编码RNA（long non-coding RNAs, lncRNAs）在前列腺癌的发生与发展进程中发挥重要作用。增强子RNA（enhancer RNA, eRNAs）与超级增强子关联长链非编码RNA（super-enhancer lncRNA, SE-lncRNAs）是长链非编码RNA的重要组成类型，但二者在前列腺癌中的具体功能仍不甚明确。本研究通过微阵列（microarray）技术，筛选得到在前列腺癌组织中差异表达的681个增强子RNA与292个超级增强子关联长链非编码RNA。我们构建了转录调控网络：即与增强子RNA相关的增强子及其靶基因共享相同的转录因子结合基序（TF binding motifs）。进一步探究了CCCTC结合因子（CTCF）在介导该转录调控网络中所发挥的作用。研究表明，增强子RNA（尤其是调控雄激素应答基因的增强子RNA）可作为预后生物标志物（prognostic biomarkers）与治疗靶点（therapy targets）的候选分子。本研究为前列腺癌的临床诊疗与治疗策略开发提供了全新视角。总体实验设计：本研究选取3例前列腺肿瘤组织及其配对的癌旁正常前列腺组织，开展表达谱检测，共获得18793个编码基因与72002个长链非编码RNA的表达数据，最终成功构建了有监督的（原文后续内容未完整呈现）。