Transcriptional characterization of Arabidopsis thaliana seeds treated with a red- or far red-light pulse. Transcriptional characterization of Arabidopsis thaliana seeds treated with a red- or far red-light pulse

The goal of this study was to compare the transcriptional profile (RNA-seq) of imbibed Arabidopsis thaliana Columbia-0 ecotype seeds that were treated with a 20 min red or far red pulse. The red-light pulse induces germination. Overall design: Col-0 seeds were sown in clear plastic boxes, each containing 10 mL of 0.8 % (w/v) agar in demineralized water. To establish a minimum and equal photo-equilibrium, seeds were imbibed for 2 hours in darkness and then irradiated for 20 min with a saturated far-red pulse (FRp, calculated Pfr/P= 0.03, 42 µmol.m-2.s-1) in order to minimize the quantities of Pfr formed during their development in the mother plant. Seeds were then stratified at 5 °C in darkness for 3 days, prior to the 20 minutes with a saturated red pulse (Rp, calculated Pfr/P= 0.87, 0.05 µmol.m-2.s-1) or FRp. Three biological replicates of each condition were collected 12 hours after the corresponding R and FR light pulses.

本研究旨在对比经20分钟红光或远红光脉冲处理的吸胀拟南芥哥伦比亚-0生态型种子的转录组谱（RNA-seq），其中红光脉冲可诱导种子萌发。总体实验设计：将哥伦比亚-0（Col-0）种子播种于透明塑料盒中，每盒加入10 mL脱矿质水中的0.8%（w/v）琼脂。为建立最低且均等的光平衡，种子先在黑暗中吸胀2小时，随后经20分钟饱和远红光脉冲（FRp，计算可得Pfr/P=0.03，光强42 μmol·m⁻²·s⁻¹）照射，以最小化母株生长过程中形成的Pfr含量。随后将种子置于5℃黑暗条件下层积3天，之后再分别施加20分钟饱和红光脉冲（Rp，计算可得Pfr/P=0.87，光强0.05 μmol·m⁻²·s⁻¹）或远红光脉冲。在对应红光（R）和远红光（FR）脉冲处理12小时后，收集每种条件下的三个生物学重复样本。