Association of CD81 T lymphocyte repertoire spreading with the severity of DRESS syndrome

T-cell receptor (TCR)-mediated cross-recognition is a major mechanism in the pathogenesis of drug reaction with eosinophilia and systemic symptoms (DRESS) syndrome. However, the characteristics of the TCR repertoire and the clinical significance of repertoire reformation throughout the course of DRESS are unknown. Here, we isolated CD41 and CD81 T-cells from peripheral blood of 8 DRESS patients at 10-day intervals and, sequenced CDR3-regions of the TCRB chain by high-throughput sequencing to analyze the dynamic reformation in the T-cell repertoire hierarchy.

T细胞受体（T-cell receptor, TCR）介导的交叉识别是伴嗜酸性粒细胞增多和系统症状的药物反应（drug reaction with eosinophilia and systemic symptoms, DRESS）综合征发病机制中的核心环节。然而，目前学界对于DRESS病程中T细胞受体库的特征，以及受体库重塑的临床意义仍不明确。本研究从8例DRESS综合征患者的外周血中，每隔10天分离CD41与CD81 T细胞，并通过高通量测序对TCRβ链的CDR3区域进行测序，以此分析T细胞受体库层级结构的动态重塑过程。