Gene expression profiling of Caco2 cells treated with Cudraflavone C. Homo sapiens

A total of 26 genes were up-regulated and 63 genes were down-regulated > 2-fold following Cudraflavone C treatment Overall design: Microarray experiments were performed in duplicate as follows: Caco2 cells were treated with 10 uM of Cudraflavone C or control vehicle (0.1% DMSO) for 48 h. Total RNA from cells was extracted using Qiagen RNA isolation kit (Qiagen, Valencia, CA, USA) according to the manufacturers’ protocol. RNA purity was examined by spectrophotometric determination at 260/280 nm. The microarray hybridizations were carried out using the GeneChip® Human Transcriptome Array 2.0 arrays.

经库德拉黄酮C（Cudraflavone C）处理后，共有26个基因上调表达，63个基因下调表达，下调幅度均超过2倍。实验设计：微阵列实验重复进行两次，具体操作如下：将Caco2细胞以10 μM库德拉黄酮C处理，对照组加入等体积的溶剂对照（0.1%二甲基亚砜，DMSO），处理时长为48小时。依照试剂盒说明书，采用Qiagen RNA提取试剂盒（Qiagen，美国加利福尼亚州巴伦西亚）提取细胞总RNA；通过260 nm/280 nm波长处的分光光度检测评估RNA纯度。微阵列杂交实验采用GeneChip®人类转录组芯片2.0（GeneChip® Human Transcriptome Array 2.0）完成。