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Data from: Sex determination in the wild: a field application of loop-mediated isothermal amplification successfully determines sex across three raptor species

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Mendeley Data2024-06-25 更新2024-06-27 收录
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https://datadryad.org/stash/dataset/doi:10.5061/dryad.4kr93
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Alignment of partial sequences of the CHD gene located in the Z-chromosome of Gyps fulvus, Milvus migrans and Neophron percnopterusDNA sequences of the CHD-Z fragments of Gyps fulvus, Milvus migrans and Neophron percnopterus used for design of LAMP primers suitable for sex determination. These sequences were obtained after Sanger sequencing of the CHD-Z fragments obtained by PCR using 2550F and 2718R primers (Fridolfsson & Ellegren 1999)Centeno-Cuadros_CHD-Z_Accipitridae.fasta

本数据集包含欧亚秃鹫(Gyps fulvus)、黑鸢(Milvus migrans)与白兀鹫(Neophron percnopterus)Z染色体上CHD基因(CHD gene)部分序列的比对结果,以及用于设计适用于性别鉴定的环介导等温扩增(Loop-Mediated Isothermal Amplification,LAMP)引物的上述三种鸟类的CHD-Z片段DNA序列。上述序列均通过使用2550F与2718R引物(Fridolfsson & Ellegren 1999)经聚合酶链式反应(Polymerase Chain Reaction,PCR)扩增获取CHD-Z片段后,再经桑格测序(Sanger sequencing)得到。数据集文件名为Centeno-Cuadros_CHD-Z_Accipitridae.fasta
创建时间:
2023-06-28
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