ANOVA for seedling dry weight.

In this research, the effect of seed halopriming with plasma activated water (PAW) on wheat germination parameters have been studied. Response surface methodology was used to investigate the effect of three factors including: 1) type of water (distilled water, 0.2 and 0.4 min/mL PAW), 2) priming time (1, 3 and 5 h), and 3) salinity of the priming solution (0, 25 and 50 mmol/L NaCl) on wheat germination parameters. The results revealed that increasing PAW levels up to 0.18 min/mL led to an increase in seed germination percentage, seedling weight (both fresh and dry), seedling length, vigor indexes A and B, and water uptake and decreasing mean germination time. Increasing the level of PAW by more than 0.18 min/mL had a negative effect on these parameters. The fresh and dry weight of the seedlings respectively at the 0.18 and 0.2 min/mL levels of PAW, in all salinity levels, had the highest value. The effect of halopriming on enhancing seedling length was more than other characteristics. The optimum ranges of PAW, salinity and priming time were 0.13 min/mL, 10.3 mmol/L and 120.22 min for germination percentage, 0.18 min/mL, 15 mmol/L and 191.24 min for seedling fresh weight, 0.15 min/mL, 13.84 mmol/L and 221.2 min for seedling dry weight, 0.33 min/mL, 24.36 mmol/L and 152.62 min for mean germination time, 0.11 min/mL, 19.03 mmol/L and 177.77 min for vigor indexes A and 0.1 min/mL, 18.1 mmol/L and 178.99 min for vigor indexes B.

本研究探讨了等离子体活化水（plasma activated water, PAW）介导的种子盐引发处理对小麦萌发相关参数的影响。本研究采用响应面法，考察了三类影响小麦萌发参数的因素，具体包括：1）水处理类型（蒸馏水、0.2与0.4 min/mL的等离子体活化水）；2）引发处理时长（1、3、5小时）；3）引发溶液盐浓度（0、25、50 mmol/L的氯化钠）。研究结果显示，当等离子体活化水浓度提升至0.18 min/mL时，种子萌发率、幼苗鲜重与干重、幼苗株高、活力指数A与B以及种子吸水量均显著提升，而平均萌发时间则显著缩短。当等离子体活化水浓度超过0.18 min/mL时，上述参数则会出现不同程度的下降。在所有盐浓度梯度下，幼苗鲜重与干重分别在等离子体活化水浓度为0.18 min/mL与0.2 min/mL时达到最高值。盐引发处理对幼苗株高的提升效果优于其余萌发相关性状。针对不同萌发指标，各因素的最优取值范围如下：萌发率对应的最优等离子体活化水浓度、盐浓度与引发时长分别为0.13 min/mL、10.3 mmol/L与120.22 min；幼苗鲜重对应的最优值分别为0.18 min/mL、15 mmol/L与191.24 min；幼苗干重对应的最优值分别为0.15 min/mL、13.84 mmol/L与221.2 min；平均萌发时间对应的最优值分别为0.33 min/mL、24.36 mmol/L与152.62 min；活力指数A对应的最优值分别为0.11 min/mL、19.03 mmol/L与177.77 min；活力指数B对应的最优值分别为0.1 min/mL、18.1 mmol/L与178.99 min。