Mice inflammatory responses to inhaled aerosolized LPS: effects of various forms of human alpha1-antitrypsin. Mice inflammatory responses to inhaled aerosolized LPS: effects of various forms of human alpha1-antitrypsin

Characterize how Balb/c mice responses to aerosolized LPS (lipopolysaccharide) alone or with intraperitoneal (i.p.) delivery of alpha1-antitrypsin (AAT) Overall design: We aimed to characterize Balb/c mice responses to aerosolized LPS alone or with intraperitoneal (i.p.) delivery of alpha1-antitrypsin (AAT). On days one, two, and three mice were exposed for 10 min to clean air or aerosol LPS (0.21 mg/ml). One hour after each challenge, animals were treated i.p. with saline or with (4 mg/kg body weight) one of the AAT preparations: native (nAAT), oxidized (oxAAT), recombinant (recAAT) or peptide of AAT (C-36). Experiments were terminated 6 h after the last dose of AATs.

本数据集旨在表征Balb/c小鼠对单独雾化脂多糖（lipopolysaccharide，LPS），或联合腹腔内（intraperitoneal，i.p.）递送α1-抗胰蛋白酶（alpha1-antitrypsin，AAT）的应答反应。实验设计概述：本研究旨在解析Balb/c小鼠对单独雾化LPS，或联合腹腔内给予AAT的生物学应答特征。实验第1、2、3天，小鼠每次暴露于洁净空气或雾化脂多糖（lipopolysaccharide，LPS，0.21 mg/ml）环境中10分钟。每次暴露刺激1小时后，对小鼠腹腔注射生理盐水，或按4 mg/kg体重剂量给予以下AAT制剂之一：天然型（native，nAAT）、氧化型（oxidized，oxAAT）、重组型（recombinant，recAAT）或AAT的C-36肽段。末次AAT给药6小时后终止全部实验。