Inhibition of lysine demethylase 6B blocks the development of ASXL1 truncation-mediated myeloid malignancies [RNA-seq]

To investigate the role of KDM6B in the pathogenesis of truncated ASXL1-mediated myeloid malignancies, we purified the Lin-cKit+ cells from bone marrow of WT, Kdm6bΔ/+, Asxl1Y588XTg, and Asxl1Y588XTg;Kdm6bΔ/+ mice and performed the bulk RNA-seq to study the dysregulated genes due to the reduction of Kdm6b in Asxl1 mutant context. RNA-seq for bone marrow Lin-cKit+ cells from WT (n=3), Asxl1Y588XTg (n=3), Kdm6bΔ/+ (n=3) and Asxl1Y588XTg;Kdm6bΔ/+ (n=3) mice.

为探究KDM6B在截短型ASXL1介导的髓系恶性肿瘤发病机制中的作用，我们从野生型（WT）、Kdm6bΔ/+、Asxl1Y588XTg及Asxl1Y588XTg;Kdm6bΔ/+小鼠的骨髓中纯化Lin-cKit+细胞，并开展批量RNA测序（bulk RNA-seq），以分析ASXL1突变背景下Kdm6b表达下调所导致的失调基因。本次测序的样本为上述四类小鼠的骨髓Lin-cKit+细胞，每组样本量均为3只：野生型（n=3）、Asxl1Y588XTg型（n=3）、Kdm6bΔ/+型（n=3）及Asxl1Y588XTg;Kdm6bΔ/+型（n=3）。