H-45 WGS vs IWGSC RefSeq v1.0 Genome Assembly

WGS reads of H-45 (http://dx.doi.org/10.1111/j.1467-7652.2012.00717.x) were aligned against the IWGSC Triticum aestivum Chinese Spring RefSeq v1.0 genome assembly using Minimap2. These BAM files (.bam) and index files (.bam.bai) are provided together with a summary of read alignment coverage bigWig files (.bam.bw). SNP variants were called from these BAM files to generate VCF files (.bam.vcf.gz) and index files (.bam.vcf.gz.tbi) and are provided together with a summary of SNP density (SNPs per 10 kbp) bigWig files (.bam.vcf.w10000_s10000.bw). VCF files contain the following filter values and corresponding meaning: PASS = high quality (Q>=30) homozygous; Het = high quality (Q>=30) heterozygous; LowQualHom = low quality (Q

H-45 的全基因组测序读段（WGS reads）经 Minimap2 软件对 IWGSC 提供的 Triticum aestivum 中国春 RefSeq v1.0 基因组组装进行比对。所生成的 BAM 文件（.bam）及其索引文件（.bam.bai）与读段比对覆盖情况的 bigWig 文件（.bam.bw）一同提供。通过对这些 BAM 文件进行 SNP 变异检测，生成 VCF 文件（.bam.vcf.gz）及其索引文件（.bam.vcf.gz.tbi），并附带 SNP 密度（每 10 kbp 的 SNP 数量）的 bigWig 文件（.bam.vcf.w10000_s10000.bw）。VCF 文件中包含以下过滤值及其对应含义：PASS = 高质量（Q≥30）纯合子；Het = 高质量（Q≥30）杂合子；LowQualHom = 低质量（Q