Epigenetic studies of HTLV-1 bZIP factor (HBZ) over-expressed mouse. Mus musculus

Transcriptional changes and epigenetic modulation by HTLV-1 bZIP factor (HBZ) in CD4+ T cells were analyzed. We used mouse primary CD4+ splenocytes in which HBZ was transduced by retrovirus vector to analyze the effect of HBZ on gene expression, histone acetylation, and the recruitment of acetylation-related factors. We performed RNA-seq to obtain gene expression and ChIP-seq to obtain genome-wide histone H3 acetylation pattern in mock- and HBZ- transduced mouse primary CD4+ T cells.

本研究对人类T细胞白血病病毒1型（HTLV-1）碱性亮氨酸拉链（bZIP）因子（HBZ）在CD4+ T细胞中诱导的转录变化与表观遗传调控效应进行了分析。我们以经逆转录病毒载体转导HBZ的小鼠原代CD4+脾细胞为实验模型，探究HBZ对基因表达、组蛋白乙酰化及乙酰化相关因子招募过程的调控作用。此外，我们分别对空白载体转导（mock）组与HBZ转导组的小鼠原代CD4+ T细胞开展RNA测序（RNA-seq）以获取基因表达谱，并通过染色质免疫共沉淀测序（ChIP-seq）绘制全基因组范围的组蛋白H3乙酰化图谱。