Targeting IDH1 as a pro-senescent therapy in high-grade serous ovarian cancer

We found that targeting IDH1 in HGSC induces senescence by altering the repressive histone landscape of E2F target genes Overall design: Ovcar3 cells were infected with control or shIDH1. Cells were selected in 3ug/mL puromycin for 7 days. RNA was isolated and RNA-seq was performed comparing control and knockdown RNA.

本研究发现，在高级别浆液性卵巢癌（High-grade Serous Ovarian Carcinoma, HGSC）细胞中靶向异柠檬酸脱氢酶1（Isocitrate dehydrogenase 1, IDH1），可通过改变E2F靶基因的抑制性组蛋白调控图谱，诱导细胞衰老。实验设计概述：将Ovcar3细胞分别用对照慢病毒及靶向IDH1的短发夹RNA（shIDH1）慢病毒感染，随后以3 μg/mL嘌呤霉素筛选7天。提取各组细胞总RNA，对对照组与IDH1敲低组的RNA进行RNA测序（RNA-seq）以开展对比分析。