Correlation of the kinetics of viral antigen and genomic RNA with restoration of normal cell homeostasis

The main objective of the studies within COCID Work Package 6 is to understand basic mechanisms by which viral replication machineries are removed from cells after pharmacological interruption of viral replication using functional as well as imaging techniques, including soft X-ray tomography. In this document, we highlight the progress in establishing the HCV replication models (replicons), the antiviral treatment chosen to eliminate viral structures from the host cells and the correlation between elimination of the viral replication machinery and restoration of a normal host cell homeostasis, using markers of HCV-induced stress. These data are essential to frame the experimental setup chosen for the imaging process required in subsequent stps of the project.COCID工作包6（Work Package 6）项下各项研究的核心目标，是借助功能检测技术与成像技术（包括软X射线断层扫描（soft X-ray tomography）），阐明经药物干预阻断病毒复制后，病毒复制机器（viral replication machineries）从宿主细胞中被清除的核心机制。本文件重点阐述了三方面的研究进展：一是HCV(丙型肝炎病毒，Hepatitis C Virus)复制模型（复制子，replicons）的构建；二是用于从宿主细胞中清除病毒结构的优选抗病毒治疗方案；三是借助HCV诱导的应激标志物，解析病毒复制机器清除与宿主细胞正常内稳态恢复之间的相关性。上述数据对于确定该项目后续阶段所需成像流程的实验设置至关重要。