GSH content and Antioxidant enzyme activities.

Mitochondrial thiol (majorly GSH) was assessed using CellTracker™ Green CMFDA fluorescent probe on flow cytometer. Data represent mean fluorescent intensity (MFI) of chloromethylfluorescein (CMF). Superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase activity of mitochondrial and cytosolic fractions were demonstrated as unit activity/min/mg protein. Level of significance is denoted as */# PPP

本实验采用CellTracker™ Green CMFDA荧光探针结合流式细胞仪，对线粒体巯基（主要为谷胱甘肽（GSH））进行检测。数据以氯甲基荧光素（CMF）的平均荧光强度（MFI）均值表示。分别测定线粒体与胞质组分中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）及谷胱甘肽还原酶的活性，结果以单位活性/分钟/毫克蛋白计。显著性水平标注为*/#（PPP）。