Lack of local vitamin D synthesis accelerates breast cancer development after Cyp27B1 gene ablation in mammary epithelium. Mus musculus

The ablated Cyp27b1 mice showed no difference in mammary development but tumorigenesis was significantly accelerated. Cell proliferation, angiogenesis, cell cycle progression and survival markers were upregulated by Cyp27b1 ablation, a pattern confirmed by microarray analysis. Overall design: Triplicate samples each of control mice and Cyp27b1 knockout mice.

Cyp27b1基因敲除小鼠的乳腺发育无显著差异，但肿瘤发生进程显著加快。细胞增殖、血管生成、细胞周期进程及存活标志物的表达水平在Cyp27b1基因敲除后出现上调，该表达特征经微阵列（microarray）分析验证。实验整体设计：对照组小鼠与Cyp27b1基因敲除小鼠各设置三份重复样本。