Genome-wide analysis reveals CLF-regulated transcripts in Arabidopsis

CURLY LEAF (CLF), the major histone methyltransferase of Polycomb Repressive Complex 2 (PRC2), modifies trimethylation of histone H3 lysine 27 (H3K27me3) and mediates dynamical chromatin repression in Arabidopsis. Here we profiled Arabidopsis transcriptomes obtained from roots, leaves, flowers and siliques of Col-0 and clf-28 plants using RNA-seq. Our analysis uncovered 3835 transcription units were up-regulated in clf-28. Compared with H3K27me3 ChIP-CHIP data, we found at least 42% of them were associated with H3K27me3. Overall design: Transcriptom profiling in roots, leaves, flowers and siliques of clf-28 plants.

卷叶基因（CURLY LEAF, CLF）是多梳抑制复合体2（Polycomb Repressive Complex 2, PRC2）的核心组蛋白甲基转移酶，可催化组蛋白H3赖氨酸27发生三甲基化修饰（H3K27me3），并介导拟南芥体内的动态染色质沉默过程。本研究采用RNA测序（RNA-seq）技术，对哥伦比亚野生型Col-0与clf-28突变体的根、叶、花及角果组织的转录组进行了测序分析。数据分析显示，clf-28突变体中共鉴定出3835个转录单位呈上调表达。将该结果与H3K27me3染色质免疫沉淀芯片（ChIP-CHIP）数据进行比对后发现，至少42%的上调转录单位与H3K27me3修饰存在关联。实验整体设计：对clf-28突变体的根、叶、花及角果组织开展转录组分析。