RNA-seq of developing Cyprinodon variegatus exposed to oil, hypoxia, or both. RNA-seq of developing Cyprinodon variegatus exposed to oil, hypoxia, or both

The objective of this project was to investigate the impacts of oil exposure in combination with environmental stressors on gene expression in developing C. variegatus. Overall design: C. variegatus from three different life stages (embryonic, ≤ 15 h post-fertilization; post-hatch, ≤ 24 h post-hatch, post-larval ≤ 4 d post-hatch) were exposed to oil, hypoxia, or both for 48 hours. Oil solutions used in exposures were derived from high energy water accommodated fractions (HEWAF) prepared using Macondo well oil following explosion of the Deepwater Horizon oil rig in 2010. Exposure hypoxia was defined as ≤ 2 mg/L of oxygen. All treatments were performed in triplicate. Following exposure, 10 larvae from each replicate were processed for RNA extraction and RNA sequencing.

本研究旨在探究石油暴露联合环境胁迫对发育阶段杂色海胆（C. variegatus）基因表达的影响。实验设计概述：选取处于三个不同发育阶段的该物种样本：受精后≤15小时的胚胎阶段、孵化后≤24小时的刚孵化阶段、孵化后≤4天的后幼虫阶段，将其分别暴露于石油、低氧或二者联合环境中，持续48小时。本实验所用石油溶液取自2010年"深水地平线"钻井平台爆炸后，以马孔多油井原油制备的高能量水容组分（high energy water accommodated fractions, HEWAF）。本次实验设定的低氧暴露条件为溶解氧浓度≤2 mg/L。所有处理组均设置三次生物学重复。暴露处理结束后，从每个重复样本中选取10只幼虫进行RNA提取与RNA测序。