代谢途径影响胰岛功能的机制研究

本数据集收集以小鼠原代胰岛为研究对象，记录了胰岛体外培养过程中的损伤变化及铁螯合剂干预效果的相关实验数据。研究采集了0h与48h培养条件下的转录组差异表达数据，以及8h、16h、24h、32h、40h、48h多个时间点的靶向类花生酸代谢组定量数据，同时开展了葡萄糖刺激胰岛素分泌（GSIS）功能检测、FerroOrange荧光法检测胞内Fe²⁺水平、铁螯合剂干预后GSIS恢复评估，以及STZ诱导糖尿病小鼠肾包膜下胰岛移植后的葡萄糖耐量测定与移植物胰岛素免疫荧光染色分析。数据由RNA测序仪、液相色谱-质谱联用仪（LC-MS）及荧光显微镜等仪器采集生成，实验在天津医科大学实验平台按标准化流程完成。

数据集共包含10个文件，总大小约300MB，其中2个CSV格式文件记录靶向代谢组学定量数据与转录组差异基因结果，8个JPG格式图像文件展示胰岛形态、Fe²⁺荧光检测、GSIS功能及移植物免疫荧光染色等实验结果。数据集主要呈现胰岛移植物体外培养过程中铁死亡标志物的动态变化，以及铁螯合剂对胰岛功能的保护作用和对移植效果的改善情况。本数据集主要面向原代胰岛体外培养损伤机制、铁死亡与胰岛功能关系及胰岛移植前处理策略优化相关研究，可供该领域研究人员参考使用。

This dataset collects experimental data using primary mouse pancreatic islets as the experimental subjects, documenting the dynamic damage changes of islets during in vitro culture and the efficacy of iron chelator intervention. The study acquired differentially expressed transcriptomic data under 0h and 48h culture conditions, as well as quantitative targeted eicosanoid metabolome data at multiple time points including 8h, 16h, 24h, 32h, 40h and 48h. Meanwhile, multiple assays were conducted: glucose-stimulated insulin secretion (GSIS) functional detection, intracellular Fe²⁺ level measurement via FerroOrange fluorescence assay, GSIS recovery evaluation after iron chelator intervention, glucose tolerance measurement and graft insulin immunofluorescence staining analysis following subrenal capsular islet transplantation in streptozocin (STZ)-induced diabetic mice. The data were acquired and generated using instruments including RNA sequencers, liquid chromatography-mass spectrometry (LC-MS) and fluorescence microscopes. All experiments were performed following standardized protocols on the experimental platform of Tianjin Medical University.

This dataset contains a total of 10 files with an overall size of approximately 300 MB. Among them, 2 CSV-format files record quantitative targeted eicosanoid metabolome data and transcriptomic differentially expressed gene results, while 8 JPG-format image files display experimental results including islet morphology, Fe²⁺ fluorescence detection, GSIS function assays and graft immunofluorescence staining. This dataset primarily showcases the dynamic alterations of ferroptosis markers during in vitro culture of islet grafts, as well as the protective effects of iron chelators on islet function and improvements to transplantation outcomes. This dataset is primarily intended for studies on the injury mechanisms of primary islets during in vitro culture, the correlation between ferroptosis and islet function, and the optimization of pre-transplantation treatment strategies for islet transplantation, and can be referenced and utilized by researchers in this field.