Analysis of the intestinal and oropharyngeal microbiome, nutritional and immunological profile of children with Autism Spectrum Disorder (ASD) compared with children without. Analysis of Intestinal and oropharyngeal microbiome of children with Autism

The present work proposes an innovative approach because in previous studies it has been the intestinal microbiota has been prioritized and the role of the microbiota of the Oropharynx in autism spectrum disorders, despite having reports from the connection between changes in the intestinal and oropharyngeal microbiota. The results of this analysis has the potential to give rise to relevant information for theinvestigation of the interconnections that influence the ASD. Changes that are evidenced in the composition and physiology of the oropharyngeal and intestinal microbiome compare the cases of ASD and controls, will allow to delimit the mechanisms metabolic, neurological and immunological that mediate and underlie the symptomatology of the ASD. Therefore, this research will focus on the study of the microbiota Oropharyngeal along with the intestinal to correlate the epidemiological data of thechildren with ASD, contrasting with the control children. The information compiled will incorporate new evidence into the scheme followed by the effector mechanisms of the neurological alterations of ASD. In this study we will investigate the diversity, composition and richness patterns of the microbiota of children with and without ASD. The data will allow us to find associations between ASD disorders with comorbidities such as gastric problems, respiratory and immunological. We will compare the information provided by parents about dietary factors, medication consumption, nutritional behaviors, other chronic diseases, care of the mother during pregnancy and other factors that can be correlated with the patterns of the microbiota found.With these data you can identify what types of bacteria are associated with the states healthy and with the decrease of the severity of the symptoms of ASD.

本研究提出了一种创新性研究思路，既往相关研究多优先关注肠道菌群，尽管已有研究报道肠道菌群与口咽菌群的变化存在关联，但口咽菌群在自闭症谱系障碍（Autism Spectrum Disorders, ASD）中的作用却长期被忽视。本分析所得结果，可为探究影响自闭症谱系障碍的菌群互作机制提供关键参考信息。通过对比自闭症谱系障碍患儿与健康对照儿童的口咽、肠道微生物组的组成与生理特征差异，可明确介导自闭症谱系障碍症状、并作为其发病基础的代谢、神经及免疫调控机制。因此，本研究将同步聚焦口咽菌群与肠道菌群，结合自闭症患儿与健康对照儿童的流行病学数据开展关联分析。本研究汇总的相关信息，将为自闭症谱系障碍神经损伤效应机制的研究框架补充新的实验证据。本研究将探究自闭症患儿与健康儿童的菌群多样性、组成及丰富度特征，基于上述数据可明确自闭症谱系障碍与共患疾病（如胃肠道疾病、呼吸系统疾病及免疫相关疾病）之间的关联。我们还将比对家长提供的各类相关信息，包括饮食因素、用药情况、营养行为、其他慢性疾病史，以及母亲孕期护理情况等可与所检测到的菌群特征建立关联的潜在影响因素。借助上述研究数据，可明确与健康状态相关、以及与自闭症谱系障碍症状严重程度降低相关的细菌类群。