Analysis of IFN-I responses of human CD1c+ DC and monocytes by CITE-sequencing. Analysis of IFN-I responses of human CD1c+ DC and monocytes by CITE-sequencing

Using CITE-sequencing, cell-type specific responses to type-I interferon stimulation were analysed across human CD1c+ dendritic cell and monocyte subsets, showing that IFN-β drives the maturation of DC3 and the acquisition of a unique costimulatory profile characterized by high GITRL expression. Overall design: PBMC from three distinct human donors were cultured for 18h with or without recombinant human IFN-β. Cells were then enriched for CD1c+ dendritic cells and monocytes, labeled with TotalSeq-A antibodies and partitioned using the 10X platform. The list of Total-seq A antibodies and their oligo tag sequence is available as a supplementary file.

本研究采用CITE测序（CITE-sequencing），对人CD1c阳性树突状细胞（CD1c+ dendritic cell）及单核细胞亚群针对I型干扰素刺激的细胞类型特异性应答进行了系统分析，结果显示干扰素-β（IFN-β）可驱动DC3细胞成熟，并使其获得以高表达糖皮质激素诱导的TNF样配体（GITRL）为特征的独特共刺激表达谱。整体实验设计：从3名不同人类供者体内分离外周血单个核细胞（PBMC），将其分为两组，分别在添加或不添加重组人干扰素-β的培养基中培养18小时。随后富集CD1c阳性树突状细胞与单核细胞，使用TotalSeq-A抗体进行标记，并通过10X平台完成细胞分区。TotalSeq-A抗体列表及其寡核苷酸标签序列可作为补充文件获取。