Reconstruction of hippocampus CA1 stratum pyramidale, pyramidal cell

title : Reconstruction of hippocampus CA1 cell morphologies

specimen : Rattus norvegicus

sex : male

weight : 100-200 g

This neuron was recorded and filled with biocytin in a 450-500 µm thick coronal slice of adult rat hippocampus, using sharp electrodes (for methods see PMID: 11807843).  The fixed slice was resectioned at 50-60 µm and the cell reconstructed with Neurolucida using a 100x oil immersion objective.

标题：海马CA1区神经元形态重构
实验动物：褐家鼠（Rattus norvegicus）
性别：雄性
体重：100~200 g
本神经元于成年大鼠海马450~500 μm厚冠状脑片上，采用锐性电极进行胞内记录并注入生物胞素（biocytin），具体实验方法详见PMID: 11807843。固定后的脑片被重切片至50~60 μm厚度，随后使用100倍油浸物镜，通过Neurolucida软件完成该细胞的形态重构。