Expression data from activated bone marrow derived macrophages from control and BRD4 conditional KO mice

Macrophages are cells belongs to innate immune system, which response to pathogen by the production of inflammatory proteins those that are effective in both combating pathogen and wound healing. Using small molecule inhibitors it has been shown that many of the inflammatory response genes were under control of BET proteins. We used LysM-Cre to conditionally delete floxed BRD4 in bone marrow derived macrophages and monitored it's effect on inflammatory gene expression Bone marrow derived macrophages were treated with either LPS, Interferon gamma and IL-4 alone or with LPS with a pre treatment of interferon gamma for RNA extraction and hybridization on Affymetrix microarray. Samples from untreated cells were used as control. To compare differential gene expression samples were prepared from wild type and BRD4 deleted macrophages. Biological duplicates were used for each treatment condition

巨噬细胞（macrophages）属于固有免疫系统细胞，可通过分泌炎性蛋白响应病原体侵染——这类蛋白兼具抗感染与促进伤口愈合的双重功能。已有研究借助小分子抑制剂证实，多数炎症应答基因受BET蛋白（BET proteins）调控。本研究采用LysM-Cre工具，在骨髓来源巨噬细胞（bone marrow derived macrophages, BMDMs）中条件性敲除两侧带有loxP位点的BRD4基因，并监测其对炎症基因表达的影响。我们将骨髓来源巨噬细胞分别单独以脂多糖（Lipopolysaccharide, LPS）、干扰素-γ（Interferon gamma, IFN-γ）及白细胞介素4（Interleukin-4, IL-4）处理，或先用干扰素-γ预处理后联合脂多糖处理，随后提取RNA并在Affymetrix基因芯片上完成杂交实验。以未处理的细胞样本作为对照。为比较差异基因表达水平，我们分别从野生型及BRD4缺失的巨噬细胞中制备实验样本。每个处理条件均设置生物学重复。