Microbes follow Humboldt: temperature drives plant and soil microbial diversity patterns from the Amazon to the Andes

Soil microbial diversity, by high-throughput sequencing data to characterise the variation in marker gene sequences, for 14 sites along a 3000 m elevation gradient in tropical forest in Peru. For bacterial community composition, the 16S rRNA gene was amplified in triplicate PCR reactions using the 515f and 806r primers. For fungal community composition, the first internal transcribed spacer region (ITS1) of the rRNA gene was amplified using the ITS1-F and ITS2 primer pair.

本数据集基于高通量测序数据表征标记基因序列的变异特征，涵盖秘鲁热带森林沿3000米海拔梯度分布的14个采样点的土壤微生物多样性信息。针对细菌群落组成分析，采用515f与806r引物对，通过三次重复PCR反应扩增16S rRNA基因（16S rRNA gene）；针对真菌群落组成分析，采用ITS1-F与ITS2引物对，扩增核糖体RNA（rRNA）基因的第一内部转录间隔区（ITS1）。