Impact of Gut Microbiome Disruption on Central and Enteric Nervous System Pathology in CLN2 Disease

This project investigates whether modulation of the gut microbiome influences central and enteric nervous system pathology in a mouse model of CLN2 disease (Tpp1R207X/R207X). Fecal samples were collected from wild-type and CLN2 mice that remained untreated or were given a VNAM broad-spectrum antibiotic cocktail. The study includes 16S rRNA gene sequencing of fecal microbiota at multiple timepoints to characterize microbial diversity and composition changes following VNAM treatment. Raw sequencing data are deposited for public access.

本研究旨在探究肠道微生物组（gut microbiome）的调控是否会影响CLN2病（CLN2 disease）Tpp1R207X/R207X小鼠模型的中枢神经系统与肠神经系统病理变化。研究收集了野生型与CLN2模型小鼠的粪便样本，受试小鼠分为未处理组与接受VNAM广谱抗生素混合制剂处理组。本研究通过多个时间点的粪便微生物群16S rRNA基因测序（16S rRNA gene sequencing），对VNAM处理后小鼠粪便微生物的多样性与群落组成变化进行表征分析，原始测序数据已公开存档并对外开放获取。