Transcriptome sequencing of b1 treated HSC-T6 cells and STAT3 knockdown HSC-T6 cells

transcriptomic sequencing of HSC-T6 cells was performed to investigate the molecular mechanisms of how Saikosaponin b1 (Ssb1) inhibited the activation of HSCs by targeting STAT3. Differentially expressed genes (DEGs) regulated by Ssb1 and STAT3 were collected and analyzed with a Venn diagram. DEGs in TGF-beta1 vs TGF-beta1 + Ssb1 group and TGF-beta1 vs TGF-beta1 + shSTAT3 group were overlapped to recognize genes regulated by Ssb1 depending on STAT3. The results showed that 110 genes were upregulated and 179 genes were downregulated both in Ssb1-treated aHSCs and TGF-beta1-challenged STAT3-KD HSC-T6 cells.

本研究对HSC-T6细胞（HSC-T6 cells）开展转录组测序（transcriptomic sequencing），旨在探究柴胡皂苷b1（Saikosaponin b1，Ssb1）通过靶向信号转导与转录激活因子3（signal transducer and activator of transcription 3，STAT3）抑制肝星状细胞（hepatic stellate cells，HSCs）活化的分子机制。研究收集并分析了柴胡皂苷b1与STAT3所调控的差异表达基因（differentially expressed genes, DEGs），并通过韦恩图（Venn diagram）进行分析；将转化生长因子β1（transforming growth factor beta 1，TGF-β1）组与TGF-β1+Ssb1组、TGF-β1组与TGF-β1+shSTAT3组的差异表达基因进行交集分析，以鉴定依赖于STAT3的柴胡皂苷b1调控基因。结果显示，在经Ssb1处理的活化肝星状细胞（activated hepatic stellate cells，aHSCs）以及经TGF-β1刺激的STAT3敲低HSC-T6细胞中，共有110个基因上调、179个基因下调。