CRISPR-Cas9 combinatorial KO of epigenetic regulators in human ovarian cancer cells

We sequenced polyA mRNA from OVCAR8-ADR-Cas9 cells in which one or two of 3 epigenetic regulators (BRD4, KDM4C, KDM6B) had been knocked out to examine how global gene expression was affected and evaluate potential synergistic effects at a molecular level. Overall design: Gene expression data (RNA-Seq) in OVCAR8-ADR-Cas9 cells infected with control vector or vectors expressing gRNAs targeting one of 4 epigenetic regulators (BRD4, KDM4C, KDM6B) with biological replicates.

本研究对OVCAR8-ADR-Cas9细胞中的聚腺苷酸信使RNA（polyA mRNA）进行测序。该细胞系中，3种表观遗传调控因子（epigenetic regulator）BRD4、KDM4C、KDM6B中的一种或两种已被敲除，本研究旨在探究其对全基因组基因表达的影响，并在分子水平评估潜在的协同效应。
实验设计概述：本研究对感染空载体对照或表达靶向4种表观遗传调控因子（BRD4、KDM4C、KDM6B）的向导RNA（gRNA）的OVCAR8-ADR-Cas9细胞进行分析，获取其基因表达数据（RNA测序（RNA-Seq）），所有样本均设置生物学重复。