Targeted genomic sequencing with probe capture for discovery and surveillance of coronaviruses in bats

This study evaluated the use of hybridization probe capture for targeted genomic sequencing of coronaviruses in oral and rectal swab specimens collected from bats in the Democratic Republic of the Congo. Probe capture identified 5 specimens with novel alpha- and betacoronaviruses. These were deep sequenced to retrieve full genomes for these novel viruses.

本研究评估了杂交探针捕获（hybridization probe capture）技术在针对刚果民主共和国蝙蝠采集的口腔与直肠拭子样本开展的冠状病毒靶向基因组测序中的应用。该探针捕获技术成功鉴定出5份携带新型α冠状病毒与β冠状病毒的样本，随后对这些样本进行深度测序，以获取这些新型冠状病毒的完整基因组序列。