Transcriptomics reveals molecular differences in equine oocytes vitrified before and after in vitro maturation

The aim of this study was to analyze transcriptome profiles with RNA sequencing (RNA-seq) in metaphase II horse oocytes after vitrification before and after in vitro maturation (IVM). To do so, transcriptome profile of three groups were compared: (1) oocytes vitrified immature, warmed, and in vitro matured (VGV), (2) in vitro matured and vitrified (VMAT), and (3) fresh oocytes (CONTROL). Overall design: Examining the effects vitrification before and after in vitro maturation on the transcriptomic level of the mature oocyte.

本研究旨在利用RNA测序（RNA sequencing, RNA-seq）分析玻璃化冷冻前后经体外成熟（in vitro maturation, IVM）处理的减数分裂II期马卵母细胞的转录组谱。为此，本研究对三组样本的转录组谱进行了比较：（1）未成熟卵母细胞经玻璃化冷冻、复苏后行体外成熟组（VGV）；（2）体外成熟后行玻璃化冷冻组（VMAT）；（3）新鲜卵母细胞对照组（CONTROL）。实验整体设计：探究体外成熟前后玻璃化冷冻对成熟卵母细胞转录组水平的影响。