Effect of SARS-CoV-2 infection on gene expression of iPSC derived sensory neurons [bulkRNAseq]

To investigate the impact of SARS-CoV-2 on the peripheral nervous system, we generated human sensory neurons from induced pluripotent stem cells (iPSC) that we further infected with the SARS-CoV-2 strain WA1/2020 for 48 hours. We then performed gene expression profiling analysis using data obtained from RNA-seq of mock treated cells compared to SARS-CoV-2 infected cells. Overall design: Comparative gene expression profiling analysis of RNA-seq data for mock treated cells compared to SARS-CoV-2 infected cells.

为探究严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）对周围神经系统的影响，我们从诱导多能干细胞（iPSC）中分化获取人感觉神经元，随后使用SARS-CoV-2毒株WA1/2020对其进行感染，感染时长为48小时。随后，我们以空白对照（mock treated）细胞与SARS-CoV-2感染细胞的RNA测序（RNA-seq）数据为基础，开展基因表达谱对比分析。整体实验设计：针对空白对照细胞与SARS-CoV-2感染细胞的RNA测序数据，完成基因表达谱的对比分析。