RNA-seq of miR-146a-5p overexpressing T24 human bladder cancer cells. RNA-seq of miR-146a-5p overexpressing T24 human bladder cancer cells

We established stable miR-146a-5p overexpression T24 cells, then performed transcriptome profiling of miR-146a-5p overexpressing cells compared to control T24 cells to detect the molecular mechanisms of the miR-146a-5p’s effect on bladder cancer cells. Overall design: T24 human bladder cells were transfected with a scramble control or with a mimic for the tumor suppressor miRNA, miR-146a-5p and following validation of miRNA expression, were subjected to gene expression profiling

本研究构建了稳定过表达微小RNA 146a-5p (miR-146a-5p)的T24细胞系，随后以转染阴性对照scramble序列的T24细胞为对照组，对miR-146a-5p过表达细胞进行转录组谱分析，以探究miR-146a-5p调控膀胱癌细胞的分子机制。实验设计概述：将人膀胱癌细胞T24分别转染阴性对照scramble序列，以及抑癌性miR-146a-5p模拟物（mimic）；在验证miRNA表达水平后，对两组细胞开展基因表达谱分析。