Additional file 1 of GIF1 controls ear inflorescence architecture and floral development by regulating key genes in hormone biosynthesis and meristem determinacy in maize

Additional file 1: Supplemental Figure 1. Identification of total proteins and immunoprecipitated proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. Supplemental Figure 2. Summary of chromatin immunoprecipitation sequencing (ChIP-seq). Supplemental Figure 3. Targets of GIF1 detected by chromatin immunoprecipitation sequencing (ChIP-seq). Supplemental Figure 4. Schematic diagram of the gif1 over-expression construct. The construct components include the T-DNA right border, RB; and left border, LB; CaMV35S promoter, CaMV35S; terminator of nopaline synthase gene, tnos; enhanced green fluorescent protein gene, eGFP; the phosphinothricin acetyltransferase cassette, bar. Supplemental Table 1. Conservation of identified proteins in Arabidopsis, maize leaf and maize ear. Supplemental. Table 2. Putative GIF1-bound targets identified by ChIP-seq and RNA-seq. Supplemental Table 3. Primer sequences used in this study.

附加文件1：补充图1。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）与免疫印迹法鉴定总蛋白及免疫沉淀蛋白。补充图2。染色质免疫共沉淀测序（ChIP-seq）概况。补充图3。通过染色质免疫共沉淀测序（ChIP-seq）检测的GIF1靶标。补充图4。gif1过表达构建体示意图。该构建体组分包括T-DNA右边界（RB）、左边界（LB）；花椰菜花叶病毒35S启动子（CaMV35S）；胭脂碱合酶基因终止子（tnos）；增强型绿色荧光蛋白基因（eGFP）；草丁膦乙酰转移酶表达盒（bar）。补充表1。拟南芥、玉米叶片及玉米穗中鉴定蛋白的保守性。补充表2。通过染色质免疫共沉淀测序（ChIP-seq）与RNA测序（RNA-seq）鉴定的GIF1潜在结合靶标。补充表3。本研究使用的引物序列。