Proteomic analysis of Antarctic sea-ice yeast Rhodotorula mucilaginosa AN5 responding to hypersalinity and hyposalinity stress

We investigated the survival and adaptive strategies of Antarctic yeast <i>Rhodotorula mucilaginosa</i> AN5 treated individually with high (15 %) and low (0 %) salinity to simulate the natural environment.We performed growth assays, SEM, biochemical factor assays, and proteomic analysis of yeast based on iTRAQ and PRM technology.

我们针对南极粘红酵母（*Rhodotorula mucilaginosa*）AN5，分别采用高盐（15%）与低盐（0%）条件单独处理以模拟其自然生存环境，进而探究其存活与适应策略。在此基础上，我们开展了该酵母的生长测定、扫描电子显微镜（Scanning Electron Microscope，SEM）观察、生化因子检测，以及基于同位素标记相对和绝对定量（isobaric Tags for Relative and Absolute Quantitation，iTRAQ）与平行反应监测（Parallel Reaction Monitoring，PRM）技术的蛋白质组学分析。