Data Sheet 1_Move contrast X-ray imaging reveals wall infiltration along maize leaf vessels before water refilling.docx

Research on the recovery mechanism of embolized vessels requires dynamic and sensitive observations of water refilling. The stable translation of the water/air interface was observed using X-ray absorption and phase contrast imaging. In this study, move contrast X-ray imaging (MCXI) was used to investigate the effect of the microstructure within microvessels on water refilling. Experimental verification using a maize leaf demonstrated that this method was approximately 12 times more sensitive than traditional transmission X-ray imaging. The pre-infiltration of water into the sieve pores of the side wall before water refilling was observed, which provided evidence of a deduction of two-step dynamic equilibrium during water refilling along cavitated vessels and water level rise in dynamic equilibrium instead of translating stably. Several quantitative analyses of the dynamic equilibrium were conducted owing to the high sensitivity of MCXI, making MCXI a promising tool to further investigate the micro-mechanism recovery of embolized vessels.

栓塞导管（embolized vessels）的恢复机制研究，需对水分复灌过程开展动态且高灵敏的观测。本研究借助X射线吸收成像与相衬成像技术，观测到水-气界面的稳定位移。本研究采用运动对比X射线成像（move contrast X-ray imaging, MCXI）技术，探究微维管内微观结构对水分复灌过程的影响。以玉米叶片开展的实验验证表明，该方法的灵敏度约为传统透射式X射线成像的12倍。研究人员观测到，在水分复灌前，水分会预先渗入侧壁的筛孔中；该现象为以下推论提供了依据：栓塞导管内的水分复灌过程存在两步动态平衡，且动态平衡下的水位上升并非稳定位移。得益于MCXI的高灵敏度，本研究对该动态平衡开展了多项定量分析，这也使得MCXI成为进一步探究栓塞导管微观恢复机制的极具潜力的工具。