Arbuscular mycorrhizal fungal communities in soybean rhizosphere soils from major producing regions of Benin

This dataset comprises Illumina-based metabarcoding reads of arbuscular mycorrhizal (AM) fungi DNA extracted from soybean rhizosphere soils collected across major soybean-producing regions of Benin. The study aimed to characterize the molecular diversity and community composition of AM fungi associated with soybean roots and to identify key environmental drivers shaping their distribution. Rhizosphere soil samples were collected from representative sites spanning different agroecological zones, with metadata including soil pH, texture, organic carbon, and climatic parameters. DNA was extracted using standard soil DNA protocols, and the AM fungal community was amplified using primers (Wanda-AML2) targeting the 18S rRNA gene region before sequencing on the Illumina MiSeq platform. The resulting data provide the first country-scale molecular reference of AM fungi communities in soybean systems from Benin.

本数据集涵盖从贝宁主要大豆产区采集的大豆根际土壤中提取的丛枝菌根（arbuscular mycorrhizal，AM）真菌DNA的Illumina平台元条形码（metabarcoding）测序读段。本研究旨在解析与大豆根系共生的AM真菌的分子多样性与群落组成，并明确调控其分布的关键环境驱动因子。研究从覆盖不同农业生态区的代表性样点采集根际土壤样本，配套记录的元数据（metadata）包含土壤pH值、质地、有机碳含量及气候参数。实验采用标准土壤DNA提取流程获取基因组DNA，使用靶向18S rRNA基因区域的引物Wanda-AML2扩增AM真菌群落，随后在Illumina MiSeq测序平台完成测序。本数据集产出的测序数据，为贝宁大豆种植体系中的AM真菌群落提供了首份国家级尺度的分子参考资料。