Islands Within Islands: Bacterial Phylogenetic Structure and Consortia in Hawaiian Lava Caves and Fumaroles

Lava caves, tubes, and fumaroles in Hawai‘i present a range of volcanic, oligotrophic environments from different lava flows and host unexpectedly high levels of bacterial diversity. These features provide an opportunity to study the ecological drivers that structure bacterial community diversity and assemblies in volcanic ecosystems and compare the older, more stable environments of lava tubes, to the more variable and extreme conditions of younger, geothermally active caves and fumaroles. Using 16S rRNA amplicon-based sequencing methods, we investigated the phylogenetic distinctness and diversity and identified microbial interactions and consortia through co-occurrence networks in 70 samples from lava tubes, geothermal lava caves, and fumaroles on the island of Hawai‘i. Our data illustrate that lava caves and geothermal sites harbor unique microbial communities, with very little overlap between caves or sites. We also found that older lava tubes (500–800 yrs old) hosted greater phylog...

夏威夷的熔岩洞穴（lava caves）、熔岩管及喷气孔（fumaroles）呈现出源自不同熔岩流的多种火山贫营养环境（oligotrophic environments），并栖居着出乎意料的高丰度细菌多样性。这些地貌特征为研究火山生态系统中细菌群落多样性与组装的生态驱动因子提供了契机，同时可将熔岩管这种更古老、更稳定的环境与年轻且地热活跃的洞穴及喷气孔中更具变异性和极端性的条件进行对比。通过基于16S rRNA扩增子的测序方法（16S rRNA amplicon-based sequencing methods），我们对夏威夷岛熔岩管、地热熔岩洞穴及喷气孔的70份样本展开研究，分析其系统发育独特性与多样性，并通过共现网络（co-occurrence networks）识别微生物相互作用及菌群。数据表明，熔岩洞穴和地热位点栖居着独特的微生物群落，不同洞穴或位点间的群落重叠度极低。我们还发现，较古老的熔岩管（500–800年）具有更高的系统发育...