Composite linkage map and enhanced genome map for Culex pipiens complex mosquitoes

We report here the development of 65 novel microsatellite loci and construction of a composite genetic linkage map for Culex pipiens complex mosquitoes. Microsatellites were identified by in silico screening of the Culex quinquefasciatus genome assembly. Cross-species utility of 73 microsatellites for population studies in C. pipiens sensu stricto and C. quinquefasciatus was evaluated by genotyping a subset of samples collected in Indiana, United States, and Point Fortin, Trinidad. Allele frequencies of 67 microsatellites were within Hardy–Weinberg expectations in both population subsets. A composite linkage map was constructed based on restriction fragment length polymorphism and microsatellite polymorphisms in 12 independent F1 intercross mapping populations. The composite map consists of 61 marker loci totaling 183.9 cM distributed across the 3 linkage groups. These loci cover 29.5, 88.8, and 65.6 cM on chromosomes I–III, respectively, and allow for assignment of 10.4% of the genome ...

本文报道了65个新型微卫星位点（microsatellite loci）的开发，以及库蚊复合群（Culex pipiens complex）蚊子复合遗传连锁图谱的构建。通过对致倦库蚊（Culex quinquefasciatus）基因组组装序列的计算机筛选（in silico screening）鉴定出微卫星位点。通过对美国印第安纳州和特立尼达岛Point Fortin采集的部分样本进行基因分型，评估了73个微卫星位点在尖音库蚊指名亚种（C. pipiens sensu stricto）和致倦库蚊种群研究中的跨物种适用性。67个微卫星位点的等位基因频率在两个种群亚群中均符合哈迪-温伯格平衡（Hardy–Weinberg expectations）。基于限制性片段长度多态性（restriction fragment length polymorphism）和微卫星多态性，利用12个独立的F1代互交作图群体构建了复合连锁图谱。该复合图谱包含61个标记位点，总长度为183.9厘摩（cM），分布于3个连锁群。这些位点分别覆盖I-III号染色体上29.5、88.8和65.6厘摩的区域，可用于定位基因组的10.4%...