Transcriptional profiling of adult mushroom body neurons and whole head extracts of Drosophila melanogaster [Timecourse_RNA_Naive_Trx]

We have analyzed transcript level changes in nuclei isolated from the adult mushroom body (MB) of Drosophila melanogaster, as well from whole-head (WH) nuclear extracts. MB and WH nuclei were isolated at five time-points across a 24-hour period. Examination of transcript levels in mushroom body (MB) and whole-head (WH) nuclei isolated from adult wild-type flies at five time-points across a 24-hour period. Genetic material was collected from pools of 50-60 fly heads with 3 replicates per condition.

我们对成年黑腹果蝇（Drosophila melanogaster）蘑菇体（mushroom body, MB）与全脑（whole-head, WH）细胞核提取物中的转录水平变化进行了分析。本研究在24小时周期内的五个时间点分离蘑菇体（MB）与全脑（WH）细胞核，并对成年野生型（wild-type）果蝇在该周期五个时间点分离得到的上述两种细胞核的转录水平开展了检测。实验所用遗传材料取自50-60头果蝇头部的混合样本，每个实验条件设置3次生物学重复。