DNA Methylation data from early human B-cell development. Homo sapiens

A global DNA methylation and gene expression analysis of early human B-cell development reveals a demethylation signature and transcription factor network. Nucleic Acids Res. 2012 Dec;40(22):11339-51. We examined DNA methylation and RNA expression status during early B-cell development by sorting multiple replicates of four separate stages of pre-B cells derived from normal human fetal bone marrow and applied high-dimension DNA methylation scanning and expression arrays. We identified a distinct development-dependent demethylation signature which has gene expression regulatory properties for pre-B cells, and provide a catalog reference for the epigenetic changes that occur in pre-B-cell leukemia and other B-cell-related diseases. Overall design: Human fetal bone marrow was obtained, and using flow cytometry antibodies, four populations of B-cell developmental stages including stage 1 (S1; predominantly multipotent progenitors before lineage commitment and common lymphoid progenitors), stage II (S2; pre-B-I cells), stage 3 (S3; pre-B-II cells) and stage 4 cells (S4; immature B cells). For 8 indibiduals, DNAs and RNAs were isolated and the methylation and expression changes during early B-cell development were identified using the Illumina HumanMethylation450 Beadchip (Illumina) and GeneChip Human Gene 1.0 ST Array (Affymetrix).

《人类早期B细胞发育的全基因组DNA甲基化与基因表达分析揭示去甲基化特征及转录因子调控网络》，发表于《核酸研究》（Nucleic Acids Research），2012年12月；40(22):11339-11351。本研究通过分选源自正常人类胎儿骨髓的四个独立前B细胞（pre-B cell）发育阶段的多个生物学重复样本，对早期B细胞发育过程中的DNA甲基化与RNA表达状态进行检测，并应用高维DNA甲基化扫描技术与基因表达芯片开展分析。本研究鉴定出一种随发育进程变化的独特去甲基化特征，该特征对前B细胞具有基因表达调控功能，并为前B细胞白血病及其他B细胞相关疾病中的表观遗传变化提供了参考数据集。实验设计：获取人类胎儿骨髓样本，借助流式细胞术抗体分选获得四个B细胞发育阶段的细胞群：1期（S1；主要为谱系定向前的多能祖细胞与共同淋巴祖细胞）、2期（S2；前B-I细胞）、3期（S3；前B-II细胞）及4期细胞（S4；未成熟B细胞）。针对8名个体，分别提取其基因组DNA与总RNA，通过Illumina HumanMethylation450 Beadchip（Illumina公司）与GeneChip Human Gene 1.0 ST Array（Affymetrix公司）芯片，鉴定早期B细胞发育过程中的甲基化与基因表达变化。