Expression data from rhesus macaque tongue epithelium

A majority of individuals infected with human immunodeficiency virus (HIV) have inadequate access to antiretroviral therapy and ultimately develop debilitating oral infections that often correlate with disease progression. Our study evaluates the potential of simian immunodeficiency virus (SIV) infected rhesus macaques to serve as a non-human primate model for oral manifestations of HIV disease. Microarrays were used to characterize changes in gene expression in the dorsal tongue epithelium that occur during chronic SIV infection. Epithelial cells were laser microdissected from dorsal tongue tissue sections from healthy uninfected macaques and macaques with chronic stage SIV infection and used for RNA extraction and hybridization on Affymetrix microarrays.

绝大多数感染人类免疫缺陷病毒（Human Immunodeficiency Virus, HIV）的个体无法获得充足的抗逆转录病毒疗法（antiretroviral therapy），最终会罹患严重的口腔感染，这类感染通常与疾病进展密切相关。本研究旨在评估感染猿猴免疫缺陷病毒（Simian Immunodeficiency Virus, SIV）的恒河猴能否作为人类免疫缺陷病毒感染口腔表现的非人灵长类动物模型。研究采用微阵列（Microarrays）技术，对慢性SIV感染过程中舌背上皮细胞的基因表达变化进行表征分析：研究人员从健康未感染恒河猴与慢性SIV感染恒河猴的舌背组织切片中，通过激光显微切割技术分离上皮细胞，将其用于RNA提取，并在Affymetrix微阵列芯片上开展杂交实验。