DataSheet1_Insights into the CD1 lipidome.docx

CD1 isoforms are MHC class I-like molecules that present lipid-antigens to T cells and have been associated with a variety of immune responses. The lipid repertoire bound and presented by the four CD1 isoforms may be influenced by factors such as the cellular lipidome, subcellular microenvironment, and the properties of the binding pocket. In this study, by shotgun mass spectrometry, we performed a comprehensive lipidomic analysis of soluble CD1 molecules. We identified 1040 lipids, of which 293 were present in all isoforms. Comparative analysis revealed that the isoforms bind almost any cellular lipid.CD1a and CD1c closely mirrored the cellular lipidome, while CD1b and CD1d showed a preference for sphingolipids. Each CD1 isoform was found to have unique lipid species, suggesting some distinct roles in lipid presentation and immune responses. These findings contribute to our understanding of the role of CD1 system in immunity and could have implications for the development of lipid-based therapeutics.

CD1亚型（CD1 isoforms）是一类MHC I类样分子（MHC class I-like molecules），可向T细胞呈递脂质抗原，并与多种免疫反应密切相关。四种CD1亚型结合并呈递的脂质组库（lipid repertoire）可能受细胞脂质组（cellular lipidome）、亚细胞微环境以及结合口袋特性等多重因素调控。本研究采用鸟枪质谱法（shotgun mass spectrometry）对可溶性CD1分子开展了全面的脂质组学分析，共鉴定出1040种脂质，其中293种存在于所有CD1亚型中。比较分析结果显示，各CD1亚型几乎可结合所有细胞脂质；其中CD1a与CD1c的脂质结合谱与细胞脂质组高度相似，而CD1b与CD1d则对鞘脂（sphingolipids）具有显著结合偏好性。研究发现每种CD1亚型均拥有独特的脂质物种，提示其在脂质呈递与免疫反应中发挥着差异化的生理功能。本研究结果有助于加深学界对CD1系统免疫功能的认知，同时可为脂质类治疗药物的研发提供重要参考。