Expression data from tocopherol deficient seedlings of Arabidopsis

Tocopherols (Vitamin E) are lipophilic antioxidants that are synthesized by all plants and are particularly abundant in seeds. Two tocopherol deficient mutant loci were used to examine how tocopherol deficiency impacts global gene expression during the critical peroid of germination and early seedling development when tocopherols are essential. vte1 lacks all tocopherols, but accumulates the tocopherol pathway intermediate DMPBQ,. vte2 which lacks all tocopherols and pathway intermediates. We used microarrays to examine the global gene expression in early seedlings and identify distinct classes of genes whose expression is affected by tocopherol deficient mutants vte1 and vte2. Keywords: genetic modification Arabidopsis seedlings were grown on media containing MS salts at 22C and RNA was extracted from 1 (1D)and 3 (3D)day old seedlings following cold treatment to break dormancy and synchronize germination (5 days at 6C) . We sought to compare the two tocopherol deficient mutants vte1 (VTE1) and vte2 (VTE2) with each other and the corresponding wild-type background Columbia-0 (COL).

生育酚（Tocopherols，又称维生素E）是一类脂溶性抗氧化剂，可由所有植物合成，且在种子中含量尤为丰富。本研究选用两个生育酚缺陷突变位点，探究在生育酚发挥关键作用的种子萌发与幼苗早期发育阶段，生育酚缺失如何影响全基因组基因表达。其中，vte1突变体无法合成任何生育酚，但会积累生育酚合成通路的中间产物DMPBQ；而vte2突变体不仅无法合成生育酚，同时也无法积累该通路的任何中间产物。本研究通过基因芯片（microarrays）检测早期幼苗的全基因组基因表达情况，以鉴定受生育酚缺陷突变体vte1与vte2影响的不同基因类别。关键词：遗传修饰、拟南芥。实验中，拟南芥幼苗接种于含MS盐的培养基中，于22℃条件下培养；先经6℃低温处理5天以打破休眠、同步萌发，随后分别收集萌发后1天（1D）和3天（3D）的幼苗并提取RNA。本研究旨在对两个生育酚缺陷突变体vte1（VTE1）、vte2（VTE2），以及对应的野生型背景株哥伦比亚-0（COL）进行两两比较与整体对照分析。