Multiplexed Mutation Rate Assessment of Msh2 Variants associated with Hereditary Nonpolyposis Colorectal Cancer

In this study, we used next generation sequencing combined with continuous culture to track the mutation rate of ~200 variants of Msh2 in Saccharomyces cerevisiae in order to determine pathogenicity.本研究结合下一代测序（next generation sequencing）与连续培养技术，对酿酒酵母（Saccharomyces cerevisiae）中约200个Msh2变异体的突变率进行追踪，以确定其致病性。