RNA-seq analyses of phase variation-dependent genes in Paenibacillus polymyxa E681

Paenibacillus polymyxa is a root-associated plant growth-promoting rhizobacterium. It was reported that many strains of P. polymyxa naturally exhibited the phenotypic variation. In the phase variation, the characteristics of the wild-type 'B' and the variant 'F' are very different in sporulation formation, motility, antibiotic ability and so on. For better understanding of the actual physiological changes, we performed RNA-seq analyses of P. polymyxa E681 to compare genome wide patterns of gene expression. As a result, we obtained 1,062 differentially expressed genes related to flagellar assembly and transport systems. Overall design: Two libraries constructed: one wild-type 'B' and one variant 'F' in P. polymyxa E681

多粘类芽孢杆菌（Paenibacillus polymyxa）是一类与植物根系相关的促生根际细菌。已有研究显示，该菌种的诸多菌株天然存在表型变异。在相变异过程中，野生型菌株'B'与变异株'F'的表型特征在孢子形成、运动能力、抗菌活性等多个维度均存在显著差异。为深入阐明其实际生理变化机制，本研究对多粘类芽孢杆菌E681开展RNA测序（RNA-seq）分析，以比较全基因组范围的基因表达模式。最终，本研究获得了1062个与鞭毛组装及转运系统相关的差异表达基因。实验整体设计：构建两个测序文库，分别对应多粘类芽孢杆菌E681的野生型菌株'B'与变异株'F'。