Pancreatic islets communicate with lymphoid tissues via exocytosis of insulin peptides. Pancreatic islets communicate with lymphoid tissues via exocytosis of insulin peptides

A difference in the transcriptomes of 8F10 T cells sourced from the NOD (8F10-NOD) or B16A (8F10-B16A) hosts was analyzed. As a result we found a number of biological pathways upregulated in the 8F10-NOD T cells including oxidative phosphorylation (OXPHOS), Myc targets, fatty acid metabolism, mTOR complex 1 (mTORC1) signaling. Overall design: T and B cell-depleted bone marrow stem cells from TCR alpha chain-deficient insulin peptide-specific 8F10 mice (CD45.2) were adoptively transferred into non-lethally irradiated NOD or B16A hosts (CD45.1). After 6 weeks of parking, the 8F10 T cells were FACS-sorted from inguinal lymph nodes of either host and subjected to RNA sequencing.

本研究分析了分别源自NOD（8F10-NOD）与B16A（8F10-B16A）宿主的8F10 T细胞的转录组差异。结果显示，8F10-NOD T细胞中存在多个上调的生物学通路，包括氧化磷酸化（oxidative phosphorylation, OXPHOS）、Myc靶标、脂肪酸代谢以及哺乳动物雷帕霉素靶蛋白复合物1（mTOR complex 1, mTORC1）信号通路。实验整体设计如下：从T细胞受体α链缺陷的胰岛素肽特异性8F10小鼠（CD45.2）中提取骨髓干细胞，经T、B细胞剔除处理后，过继转移至经非致死剂量辐照的NOD或B16A宿主（CD45.1）体内。饲养6周后，从两种宿主的腹股沟淋巴结中通过荧光激活细胞分选（Fluorescence Activated Cell Sorting, FACS）分离得到8F10 T细胞，随后进行RNA测序。