Genome sequence of Streptomyces nigrescens HEK616. Streptomyces nigrescens strain:HEK616

Streptomyces nigrescens HEK616 is a Gram-positive filamentous actinobacteria that possess 8.25 Mb linear genome with GC content of 71.9% and 1.07 Mb linear plasmid with GC content of 70.1%. Strain HEK616 was isolated from soil collected at Hegura Island (Ishikawa, Japan). Strain HEK616 produces antibacterial streptoaminals as secondary metabolites. Strain HEK616 also produces antifungal 5-alkyl-1,2,3,4-tetrahydroquinolines specifically when combined-culture with mycolic acid-containing bacteria. Sequence reads of Strain HEK616 was obtained by single-molecule real-time sequencing (PacBio RSII) and illumine pair end 150 bp short reads (Hiseq). Assembly was performed by HGAP4 (ver 4.0), Falcon (ver 0.3) or Canu (ver 0.7) software for PacBio reads and then Arrow (v2.3.3) / Pilon (v1.22) were used to recorrect the assembly result.

黑变链霉菌（Streptomyces nigrescens）HEK616是一株革兰氏阳性丝状放线菌，其携带一条大小为8.25 Mb的线性基因组，GC含量为71.9%，同时包含一条1.07 Mb的线性质粒，GC含量为70.1%。该菌株分离自日本石川县羽咋岛的土壤样本。HEK616可产生抗菌次级代谢产物链霉胺类（streptoaminals）；当与含分枝菌酸的细菌共培养时，该菌株还会特异性合成具有抗真菌活性的5-烷基-1,2,3,4-四氢喹啉类化合物。该菌株的序列读段通过单分子实时测序（single-molecule real-time sequencing，PacBio RSII）与Illumina双端150 bp短读段测序（HiSeq）获得。针对PacBio读段的基因组组装采用HGAP4（v4.0）、Falcon（v0.3）或Canu（v0.7）软件完成，随后使用Arrow（v2.3.3）与Pilon（v1.22）对组装结果进行校正。