Burkholderia isolates collected from a chronically infected cystic fibrosis patient over a period of 3.5 years, until the patient's death. Burkholderia isolates

Eleven Burkholderia cenocepacia clonal variants were collected from a single chronically infected cystic fibrosis patient, from the beginning of the infection until the patient's death with cepacia syndrome 3.5 years later. One Burkholderia multivorans co-infecting isolate from the same patient was also added to the study. The isolates were sequenced using Illumina, with a higher depth of coverage for 4 isolates. These longitudinal isolates have been thoroughly characterised phenotypically and at the level of the proteome, transcriptome and virulence. Coupled with detailed information on the patient's condition, disease progression and treatment management, the genomic analysis is aimed at identifying potentially adaptive mutations during chronic infection. This work is the result of a collaboration between the Institute for Bioengineering and Biosciences (iBB), Instituto Superior Técnico, Portugal, and the Cystic Fibrosis Centre of Hospital Santa Maria in Lisbon, Portugal.

本研究从1例慢性感染囊性纤维化（cystic fibrosis）患者体内采集了11株洋葱伯克霍尔德菌（Burkholderia cenocepacia）克隆变异株，采样时段覆盖感染起始至3.5年后患者因伯克霍尔德菌综合征死亡的全过程。本研究同时纳入了该患者体内分离得到的1株共感染多噬伯克霍尔德菌（Burkholderia multivorans）菌株。所有分离菌株均采用伊卢米纳（Illumina）测序平台完成测序，其中4株菌株的测序覆盖深度更高。上述纵向分离菌株已在表型、蛋白质组（proteome）、转录组（transcriptome）以及毒力层面完成了全面表征。结合患者临床状态、疾病进展与治疗管理的详细信息，本次基因组分析旨在鉴定慢性感染过程中潜在的适应性突变。本研究由葡萄牙里斯本高等技术学院生物工程与生物科学研究所（Institute for Bioengineering and Biosciences, iBB）与葡萄牙里斯本圣玛丽亚医院囊性纤维化中心合作完成。