Novel O-GlcNAcylation on Thr71 of histone H4 preferentially localizes to active gene regions

We report genome-wide distribution of newly discovered O-GlcNAcylated H4 at threonine 71 (H4T71Gc) in mouse embryonic stem cells (mESCs, J1 line) cultured in 25 mM glucose (mESCs-25mMG) or 1 mM glucose (mESCs-1mMG) condition, human embryonic stem cells (hESCs, H9) and human induced-pluripotent stem cells (hiPSCs, 201B7). We found that H4T71Gc preferentially localize to gene regions, especially those with relatively high expression levels. Interestingly, the localized gene region of H4T71Gc changes in response to changes in extracellular glucose concentration. This study using ChIP-seq analysis provides genomic distribution of novel histone O-GlcNAcylation in mouse and human pluripotent stem cells. ChIP-seq analysis of H4T71Gc in mESCs, hESCs and hiPSCs.

本研究报道了新发现的苏氨酸71位O-连接N-乙酰葡糖胺修饰组蛋白H4（H4T71Gc）的全基因组分布数据，相关样本包括分别在25毫摩尔葡萄糖（mESCs-25mMG）及1毫摩尔葡萄糖（mESCs-1mMG）培养条件下培育的小鼠胚胎干细胞（mESCs，J1细胞系）、人类胚胎干细胞（hESCs，H9细胞系）以及人类诱导多能干细胞（hiPSCs，201B7细胞系）。研究结果显示，H4T71Gc优先定位于基因区域，尤其是表达水平相对较高的基因区段。值得注意的是，H4T71Gc的定位基因区段会随细胞外葡萄糖浓度的改变发生动态变化。本研究通过染色质免疫共沉淀测序（ChIP-seq）分析，获取了小鼠与人类多能干细胞中新型组蛋白O-连接N-乙酰葡糖胺修饰的基因组分布信息，相关数据涵盖mESCs、hESCs及hiPSCs中H4T71Gc的ChIP-seq分析结果。