Nasopharyngeal carcinoma cell lines (copy number analysis)

Five NPC cell lines (HONE1, CNE1, CNE2, 5-8F, and 6-10B) and an immortalized nasopharyngeal epithelial cell line NP69 were evaluated with 250K SNP arrays (Affymetrix) to detect the genome-wide DNA copy number alterations. The same type of data of 45 Chinese samples from the HapMap project was used as normal control. Data was analyzed using GCOS (GeneChip operating software, Ver.1.4), GTYPE (GeneChip Genotyping Analysis Software, Ver.4.1), and CNAT (chromosome copy number analysis tool, Ver.4.0, BRLMM algorithm).

本研究采用Affymetrix公司的250K单核苷酸多态性（Single Nucleotide Polymorphism, SNP）芯片，对5株鼻咽癌（Nasopharyngeal Carcinoma, NPC）细胞系（HONE1、CNE1、CNE2、5-8F及6-10B）及1株永生化鼻咽上皮细胞系NP69开展全基因组DNA拷贝数变异检测。本研究同时纳入来自国际人类基因组单体型图计划（Haplotype Map Project, HapMap）的45例中国人群样本的同类型数据作为正常对照。所有数据均通过GCOS（基因芯片操作软件，版本1.4）、GTYPE（基因芯片基因分型分析软件，版本4.1）及CNAT（染色体拷贝数分析工具，版本4.0，搭载BRLMM算法）完成分析。